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. 2018 Oct 17;4(12):4311–4320. doi: 10.1021/acsbiomaterials.8b00894

Figure 5.

Figure 5

Salivary phenotypic marker and stem cell marker changes at the protein level after organization induction. The protein translation of the salivary epithelial (α-amylase, AQP5, E-cadherin, ZO-1, CK7, and CK18) or stem cell-related (OCT4, SOX2, NANOG, LGR5, CD90, and KRT5) markers was examined by Western blot, and the expression levels relative to β-actin were calculated. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean (n = 3). One-way ANOVA, Tukey’s post hoc test. *, compared with 2D plastic; #, compared with floating culture; $, compared with Matrigel. ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001, $P < 0.05, $$P < 0.01.