Table 3.

Technical validation data of the SPARC-M ELISA assay.

Tecnical validation step	SPARC-M performance
Detection range (LLOD-ULOD)	2.7–300.7 ng/mL
Intra-assay variation	6%
Inter-assay variation	10%
Dilution of serum samples	1:4
Dilution recovery (1:4 pre-dilution)	96% (77–102%)
Freeze/thaw recovery (4 cycles)	92% (86–103%)
Analyte stability up to 48 h, 4°C and 4 h, 20°C	88% (84–96%)
Interference Lipids, low/high	96%/97%
Interference Biotin, low/high	96%/98%
Interference Hemoglobin, low/high	96%/80%

Percentages are reported as mean with range shown in brackets