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. 2018 Dec 19;7:e40476. doi: 10.7554/eLife.40476

Figure 1. ICV injection of senktide stimulates LH release in female Kiss1 KO mice supplemented with estradiol.

Mean ± SEM LH concentrations (ng/ml) in male (A) and (C) and female (B) and (D) adult WT and homozygous Kiss1Cre/Cre (i.e., Kiss1 KO) mice. Blood samples were collected before (basal) and 25 min after ICV injection of senktide (an NK3R-specific agonist; 600 pmol diluted in 5 µl 0.9% NaCl). (A, B) Mean ± SEM LH concentrations (ng/ml) in adult WT male and female mice gonadectomized (WTORX or WTOVX, respectively) and studied in parallel to hypogonadal (with low sex steroid levels) Kiss1Cre/Cre (i.e., Kiss1 KO) littermates (n = 7–10/group). (C, D) Mean ± SEM LH concentrations (ng/ml) in adult gonadectomized WT and Kiss1Cre/Cre (i.e., Kiss1 KO) male and female mice supplemented with testosterone or estradiol, respectively (WTORX+T, WTOVX+E2, Kiss1 KO+T, Kiss1 KO+E2; n = 5–10/group). Basal versus after senktide injection LH concentrations were compared with a paired t-test ****p < 0.0001, **p < 0.007. T = testosterone, E2 = estradiol.

Figure 1.

Figure 1—figure supplement 1. Expression profile of (A) Kiss1 gene in the mediobasal hypothalamus (MBH), (B) Kiss1 gene in the preoptic area (POA), of ovariectomized (OVX) WT and hypogonadal Kiss1 KO female mice.

Figure 1—figure supplement 1.

Comparison between groups was carried out with a student’s t-test (*p < 0.001). The data were normalized using L19 primers as an internal control.