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. 2018 Dec 13;11:8815–8822. doi: 10.2147/OTT.S167902

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© 2018 Wang et al. This work is published and licensed by Dove Medical Press Limited

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TSPAN7 overexpression promotes cell proliferation.

Notes: (A) MTT assay was used to determine the cell proliferation rate of the A549 and H1299 cells with or without overexpression of TSPAN7 at the time point as indicated (TSPAN7-OV means TSPAN7 overexpression). (B) Effect of TSPAN7 overexpression on colony formation was measured in A549 and H1299 cells. The cells were seeded into six-well plates and cultured for 14 days, followed by crystal violet staining. The colony counts were shown below the graph. (C and D) A transwell assay was performed with the A549 and H1299 cells to measure the migration rate (points = means of three independent experiments; error bars = SEM; n=3; two-way ANOVA) (TSPAN7-OV means TSPAN7 overexpression). Statistical significance was calculated using Student’s t-tests when only two groups were compared; ^**P<0.01 and ^***P<0.001.