Table 1.

List of PCR primers for 16s ribosomal target regions and qRT–PCR

Gene name	Primer sequence 5ʹ to 3ʹ
P. aeruginosa oprL – forward	5ʹ-ATGGAAATGCTGAAATTCGGC-3ʹ
P. aeruginosa oprL – reverse	5ʹ-CTTCTTCAGCTCGACGCGACG-3ʹ
P. aeruginosa oprL – probe	(6FAM)-5ʹ-TGCGATCACCACCTTCTACTTCGAGT-3ʹ-MGBNFQ
Total 16S rDNA – forward	5ʹ-TCCTACGGGAGGCAGCAGT-3ʹ
Total 16S rDNA – reverse	5ʹ-GGACTACCAGGGTATCTAATCCTGTT-3ʹ
Total 16S rDNA – probe	(6FAM)-5ʹ-CGTATTACCGCGGCTGCTGGCAC-3ʹ-MGBNFQ
FabD – forward	5ʹ-GGTCCAGAATGGTCCTGAAGAG-3ʹ
FabD – reverse	5ʹ-CGATCGAAACCGTAAGGATGGC-3ʹ
PvdS – forward	5ʹ-GATAACCGTACGATCCTGGTGAAG-3ʹ
Pvds – reverse	5ʹ-GACGATCTGGAACAGGTAGCTGAG-3ʹ
Alg8 – forward	5ʹ-CAAGGATTTCATCCTGCTTATCGG-3ʹ
Alg8 – reverse	5ʹ-GAAACTGGTGACCATCAGGAACA-3ʹ
AlgE – forward	5ʹ-GACAACCTCGACAAGACCTATACC-3ʹ
AlgE – reverse	5ʹ-CTCGAACGATAGTTGTAGGCATCG-3ʹ