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. 2018 Dec 13;9:2806. doi: 10.3389/fimmu.2018.02806

Figure 2.

Figure 2

Binding of XCL1 and the structural variants Del-N7, Del-C7, Del-C17, and vCterm to XCR1 expressed on primary dendritic cells. Splenocytes were incubated with carefully titrated (0.04, 0.08, 0.16, 0.315, 0.625, 1.25, and 2.5 μg/ml) concentrations of all XCL1-OVA protein variants (all concentrations are given based on the XCL1-component of the constructs) for 25 min on ice, washed, and the bound protein was detected on Ly6G/C− CD3− B220− CD8+ CD11c+ MHC-II+ cells using an anti-Strep-tag mAb StrepMAB-Immo and flow cytometry (red histograms). Background staining, without pre-incubation, using StrepMAB-Immo is shown in gray. (A) Signals obtained with XCL1-variants at 0.04, 0.16, 0.625, and 2.5 μg/ml. (B) Graphical representation of the mean fluorescence intensity (MFI) obtained in flow cytometry with all protein concentrations of the XCL1-OVA variants used. (C) Signals (shown for 0.625 and 2.5/μg/ml) obtained on CD8+ DC lacking XCR1. Data are representative of 3 independent experiments.