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. 2018 Dec 13;9:2806. doi: 10.3389/fimmu.2018.02806

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Copyright © 2018 Kroczek, Hartung, Gurka, Becker, Reeg, Mages, Voigt, Freund and Kroczek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of cytotoxic capacity after in vivo targeting of antigen with XCL1-OVA and its variants Del-N7, Del-C7, Del-C17, and vCterm. C57BL/6 mice were immunized with the indicated amounts of wt XCL1-OVA and its variants (based on the content of OVA) together with 3 μg LPS on day 0. On day 6, the animals were injected with CFSE-labeled target cells to quantitate the induced cytotoxicity in vivo (for details, see Materials and Methods). (A) Exemplary flow cytometry histograms of the data obtained with 0.3 μg of each reagent. (B) Percentage of specific lysis obtained with 0.3, 1 and 3.3 μg of each reagent (mean ± SEM).