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. 2018 Jul 13;44(2):344–355. doi: 10.1038/s41386-018-0151-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Morphology of ventral midbrain and telencephalic astrocytes. a Coronal brain section from a P60 Aldh1L1-eGFP mouse immunostained for Aldh1L1 (red). b Images taken from P60 Aldh1L1-eGFP mouse section showing density and tissue coverage by eGFP+ astrocyte processes in the ventral tegmental area (VTA), cortex (CTX), and hippocampus (HPC). c Closeup of individual eGFP+ astrocytes. d Quantification of eGFP+ astrocyte density in P60 animals. One-way ANOVA, F = 0.6611, p = 0.5441, N = 5 animals, 1 section per animal, per region. e Quantification of percent tissue coverage by eGFP+ astrocyte processes in P60 animals (see Methods for details). One-way ANOVA, F = 7.866, p = 0.0025; Bonferroni post hoc tests for CTX vs HPC t = 0.1441, CTX vs VTA t = 3.536, HPC vs VTA t = 3.396 (**p < 0.01). N = 8–9 sections, 4–5 animals per region. f Quantification of eGFP+ astrocyte soma size in P60 animals. One-way ANOVA, F = 19.52, p < 0.0001; Bonferroni post hoc tests for CTX vs HPC t = 1.899, CTX vs VTA t = 4.179, HPC vs VTA t = 6.078 (***p < 0.0001). N = 6–8 sections, 4–6 animals per region. g Example scatter plot of one FACS experiment, with forward scatter values on the x-axis and FITC channel intensity on the y-axis. Circle indicates eGFP+ astrocyte considered in analysis. h Forward scatter values from one FACS experiment in which Aldh1L1-eGFP tissue microdissected from ventral midbrain, CTX, and HPC were dissociated into single-cell suspensions and analyzed by flow cytometry. Graph shows aggregate forward scatter values of eGFP+ astrocytes from the three regions. i Quantification of average forward scatter values (per animal/experiment) of eGFP+ astrocyte from ventral midbrain, CTX, and HPC. One-way ANOVA, F = 31.06, p < 0.0001; Bonferroni post hoc tests for CTX vs HPC t = 0.5599, CTX vs ventral midbrain t = 0.7089, HPC vs ventral midbrain t = 6.529 (***p < 0.001). N = 6 animals/experiments