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. 2018 Dec 19;9:5385. doi: 10.1038/s41467-018-07796-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Schematic of the T6SS apparatus, construct design, expression, and purification of the EAEC TssK_SN-TssF–TssG complex. a Schematic of the T6SS apparatus. TssE is rendered semi-transparently owing to uncertainty in its position. TssA is not shown. b Schematic representation of the construct used for recombinant expression. c Size-exclusion chromatogram after affinity purification. d SDS-PAGE of the fraction indicated with an arrow in c. Molecular weights from the ladder are indicated on the left. Uncropped gel is shown in Supplementary Figure 3