Fig. 7.
Model of the T6SS baseplate and needle complex architecture before sheath contraction. a A model was obtained by rigid-body docking subcomplex structures into the single particle cryoEM reconstruction of the non-contractile sheath mutant of the Vibrio Cholerae T6SS baseplate/needle at 8 Å resolution (EMD 3879)45. b Cut-away view of the model shown in a. c Orthogonal view of the model shown in a. d Circularization of the (TssF)2-(TssG)1 complex in the context of the T6SS baseplate. TssK, TssF, TssG are colored identically to Fig. 1; TssB–TssC: teal (PDB ID 3j9g)19; Hcp: magenta (PDB 5OJQ); VgrG-PAAR: green-olive (PDB ID 4MTK and 4JIV)9