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. 2018 Dec 19;9:5378. doi: 10.1038/s41467-018-07620-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — SRPK1 inhibition has no lasting effects on normal hematopoiesis. a Quantitation of LSK (Lin⁻/Sca1⁺/Kit⁺) and HSC (LSK/CD150⁺/CD34⁻) compartments in bone marrow from WT mice three weeks after treatment with vehicle or SPHINX31 (2 mg/kg). b Colony-forming assay of WT lineage negative (Lin−) HSPCs during (plating 1) and after (platings 2 & 3) treatment with 3 μM SPHINX31 (mean ± s.d., n = 3). c Colony-forming efficiency of CD34+ human cord blood cells (n = 4) in the presence of 1.5, 3, or 6 uM SPHINX31 (mean ± s.d., n = 4). These changes are not significant at the 95% confidence level according to one-way Anova on repeated measures. Error bars refer to variation across 4 different individuals (blue circle, brown square, red triangle, and green triangle). d Colony-forming efficiency of primary human MLL-X AML cells treated with 1.5, 3, or 6 μM SPHINX31 or 1 μM iBET-151 (mean ± s.d., n = 3 technical replicates). e Colony-forming efficiency of primary human MLL-WT AML cells treated with 1.5, 3, or 6 μM SPHINX31 or 1 μM iBET-151 (mean ± s.d., n = 3 technical replicates). HSC, hematopoietic stem cells; CFU, colony forming units; n.s., not significant; *p < 0.001 (t-test)