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. 2018 Dec 18;4(12):e01039. doi: 10.1016/j.heliyon.2018.e01039

Fig. 5.

Fig. 5

B7-H1 was associated with DNA-PKcs in cancer cells. (A) Immunoprecipitation (IP) and Western blot (WB) identified an association of B7-H1 with DNA-PKcs in B7-H1/624mel cells and B7-H1 positive Karpas 299 cells. The specificity of IP antibody to B7-H1 (clone 5H1-A3) was confirmed by WB with another antibody to B7-H1 (clone E1L3N). (B) The association of B7-H1 with DNA-PKcs was identified in MDA-MB-231 cells and this association was abolished by NU7026 (1 μM), an inhibitor of DNA-PKcs. The whole gel images of (A) and (B) were provided as the supplemental materials. (C) Co-localization of B7-H1 and DNA-PKcs in B7-H1-GFP transfected MDA-MB-231 cells before and after treatment with cisplatin (40 μM) for 72 hours. Co-localizations of B7-H1 and DNA-PKcs were identified in the nuclei. Arrowhead and arrow indicated B7-H1 at nuclear areas and co-localization with DNA-PKcs. (D) Z-section images (0.11 μm of depth per section at a range of -1, 0, +1) showed the association of DNA-PKcs (red) and B7-H1-GFP (green) within a nucleus upon treatment with cisplatin as in (C).