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. 2018 Dec 18;4(12):e01039. doi: 10.1016/j.heliyon.2018.e01039

Fig. 7.

Fig. 7

Overexpression of B7-H1 enhanced the activation of MAPK/ERK pathway in melanoma cells. (A) The relative phosphorylation levels of proteins in the MAPK/ERK pathway were measured by antibody arrays in B7-H1/624mel cells and Mock/624mel cells. (B–C) The levels of phospho-ERK1/2 and total ERK were analyzed by Western blotting (B) and flow cytometry (C). Numbers are mean fluorescence intensity (MFI). **P < 0.01 compared between Mock and B7-H1/624mel cells. The whole gel images of (B) were provided as the supplemental materials. (D) Dot plot graph shows the changes of phosphor-ERK1/2 induced by NU7026 (10 μM, 24 hours). Numbers of independent experiments (n = 4 in Mock/624mel; n = 3 in B7-H1/624mel). *P < 0.05 compared between % changes of phospho-ERK1/2 in Mock/624mel and B7-H1/624mel cells. Right panel shows the representative expression of phospho-ERK1/2 measured by flow cytometry. (E) Survivin expression increased in B7-H1/624mel cells in comparison with Mock/624mel (*P < 0.05). (F) NU7026 (10 μM) inhibited the expression of Survivin in B7-H1/624mel cells and Mock/624mel cells after 24 hours of culture. Numbers are percentages of change from control groups.