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. 2018 Dec 19;87(1):e00651-18. doi: 10.1128/IAI.00651-18

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Copyright © 2018 Bechelli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — R. australis induces an autophagy-related response instead of active autophagy in Atg5^flox/flox BMMs but not in Atg5^flox/flox Lyz-Cre BMMs. BMMs were isolated from Atg5^flox/flox Lyz-Cre and Atg5^flox/flox mice and then infected with R. australis at an MOI of 5. (A) Induction of autophagosomes by rickettsiae in these infected macrophages was evaluated by determination of the expression levels of LC3-II and p62/SQSTM1 at different time points. The data shown are the mean ± standard deviation (SD) from three independent experiments. (B) The ratios of LC3-II/ACTB and SQSTM1/ACTB in uninfected and infected Atg5^flox/flox BMMs were analyzed by densitometry. (C) Representative confocal microscopic images of infected BMMs of Atg5^flox/flox and Atg5^flox/flox Lyz-Cre mice at 1 h p.i. Green, LC3⁺ puncta; blue, DAPI (nuclei). (D) The average number of LC3 puncta per 10 cells was counted. More than 150 cells from 15 randomly selected images were counted. *, P < 0.05; n.s., not significantly different.