FIG 2.

Transcription of the bbc loci is affected by Mip and PpiB. Twenty nanograms of total RNA isolated from each strain grown on low-agar BCYE plates was subjected to qRT-PCR. As assessed by the transcript level normalized to that for the wild type, a significant decrease in the transcript level for both surfactant biosynthesis operons was measured in the Δmip ΔppiB mutant. Shown are the mean and standard deviation of three independent measurements from at least two separate RNA isolations. Statistical significance was calculated using an unpaired Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01).