FIG 3.
Growth of different L. pneumophila PPIase-negative mutants at suboptimal temperatures. Bacteria grown in liquid culture were spotted in increasing 10-fold dilutions onto BCYE agar starting at 109 bacteria/ml and grown at 37°C (A), 30°C (B), 17°C (C), or 44°C (D). Shown are representative pictures from three separate experiments performed in triplicate. Representative sections of bacterial plates from three separate experiments are shown and aligned for a better comparison of the strains. Single sections originating from different plates are separated by white dashed lines.