FIG 5.

Replication of PPIase mutant strains in human lung tissue explants (HLTEs) and THP-1 macrophages. (A) HLTEs were infected with the wild-type strain (WT) and the ΔdotA, Δmip, ΔppiB, and Δmip ΔppiB (ΔΔ) L. pneumophila mutants. Shown are the means and standard deviations from experiments performed in duplicate with tissue from five different donors. (B) Infection of THP-1 macrophages with wild-type, ΔdotA, Δmip, ΔppiB, and Δmip ΔppiB (ΔΔ) L. pneumophila strains. PPIase mutants showed decreased intracellular growth. Shown are the means and standard deviations from three experiments performed in triplicate. Statistical significance was calculated by an unpaired Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; n.s., not significant).