TABLE 1.
Wild-type strains and mutant L. pneumophila strains used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| L. pneumophila Corby | Wild-type strain | 61 |
| L. pneumophila Corby ΔdotA | dotA::Tn5 mutant of L. pneumophila Corby | Kindly provided by Antje Flieger, Robert-Koch-Institut, Wernigerode, Germany |
| L. pneumophila Corby Δmip | mip::nptI mutant of L. pneumophila Corby | 40 |
| L. pneumophila Corby ΔppiB | ppiB::nptI mutant of L. pneumophila Corby | This study |
| L. pneumophila Corby Δmip ΔppiB (ΔΔ) | mip::nptI and ppiB::cat mutant of L. pneumophila Corby | This study |
| L. pneumophila Corby ΔppiA | ppiA::aac1 mutant of L. pneumophila Corby | This study |
| L. pneumophila Corby ΔppiA ΔppiB | ppiA::aac1 and ppiB::nptI mutant of L. pneumophila Corby | This study |
| Complemented strains | ||
| WT(+) | Wild-type strain carrying empty pBCKS plasmid | This study |
| Mip(+) | mip::nptI mutant of L. pneumophila Corby carrying pEWM103 | 40 |
| PpiB(+) |
ppiB::nptI mutant of L. pneumophila Corby carrying pJRA1 ppiB
cloned in pBCKS with SacI and KpnI |
This study |
| pBCKS complementation vector | pUC ori Cmr (cat) | Stratagene (62) |