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OxyR binds the promoters of ahp and kat. (A to D) PCR-amplified promoter regions for A1S_1201 (ahpF1) (A, C, and D) or A1S_3382 (katE) (B) were incubated with purified recombinant His6-OxyR alone (A and B) or with WT His6-OxyR or His6-OxyR C202S (C and D). The protein was preincubated for 10 min with or without 50 mM H2O2. DNA was visualized using SYBR green. Each gel is representative of experiments performed on at least two separate days.
