TABLE 1.
Genes with increased expression in WT A. baumannii following treatment with H2O2
| Category and locus | Annotation (KEGG)a | Fold change |
|---|---|---|
| Peroxide detoxification | ||
| A1S_1459 | Alkyl hydroperoxide reductase subunit F (ahpF2) | 61.9 |
| A1S_1200 | Alkyl hydroperoxide reductase subunit F (ahpF1) | 49.3 |
| A1S_1458 | Alkyl hydroperoxide reductase subunit F (ahpF2) | 47.2 |
| A1S_1201 | Alkyl hydroperoxide reductase subunit F (ahpF1) | 40.3 |
| A1S_1460 | Alkyl hydroperoxide reductase subunit F (ahpF2) | 35.7 |
| A1S_3382 | Catalase (katE) | 27.7 |
| A1S_2863 | Putative antioxidant protein | 5.9 |
| Sulfur homeostasis | ||
| A1S_2533 | Putative esterase | 459.7 |
| A1S_2532 | Sulfate transport protein | 364.5 |
| A1S_2531 | Sulfate transport system substrate-binding protein | 334.4 |
| A1S_2534 | Sulfate transport system permease protein | 172.9 |
| A1S_0030 | Sulfonate transport system substrate-binding protein | 165.8 |
| A1S_0028 | Alkanesulfonate monooxygenase | 113.7 |
| A1S_2535 | Sulfate transport system permease protein | 106.1 |
| A1S_0029 | Sulfonate transport system substrate-binding protein | 71.2 |
| A1S_0027 | Sulfonate transport system permease protein | 49.6 |
| A1S_3207 | Sulfate transport system substrate-binding protein | 46.0 |
| A1S_1408 | Putative rhodanese-related sulfurtransferase | 25.5 |
| A1S_2536 | Sulfate transport system ATP-binding protein | 18.1 |
| A1S_0977 | Arylsulfatase | 18.0 |
| A1S_3306 | Dimethylsulfone monooxygenase | 17.9 |
| A1S_0026 | Sulfonate transport system ATP-binding protein | 16.2 |
| A1S_2846 | Sulfite reductase (NADPH) hemoprotein beta-component | 5.6 |
| Iron homeostasis | ||
| A1S_2389 | Iron complex transport system permease protein | 26.2 |
| A1S_2381 | 2,3-Dihydroxybenzoate-AMP ligase | 21.8 |
| A1S_1719 | 4Fe-4S ferredoxin iron-sulfur binding | 21.4 |
| A1S_2380 | Bifunctional isochorismate lyase | 18.2 |
| A1S_2379 | Histidine decarboxylase | 16.3 |
| A1S_2382 | BasD | 16.0 |
| A1S_2388 | Iron complex transport system permease protein | 15.8 |
| A1S_2387 | Iron complex transport system ATP-binding protein | 14.9 |
| A1S_2390 | Putative acinetobactin biosynthesis protein | 8.7 |
| A1S_2372 | Isochorismate synthase | 8.2 |
| A1S_2386 | Iron complex transport system substrate-binding protein | 7.4 |
| A1S_2383 | Putative acinetobactin biosynthesis protein | 6.9 |
| A1S_2392 | Putative acinetobactin utilization protein | 6.1 |
| A1S_2077 | Putative outer membrane porin receptor for Fe(III)-coprogen, Fe(III)-ferrioxamine B, and Fe(III)-rhodotorulic acid uptake (fhuE) | 5.7 |
| A1S_3339 | Iron complex outer membrane receptor protein | 5.4 |
| A1S_1607 | Iron complex outer membrane receptor protein | 4.6 |
| A1S_1647 | Putative siderophore biosynthesis protein | 4.4 |
| A1S_1359 | Iron(III) transport system substrate-binding protein | 4.3 |
| A1S_2530 | 4-Amino-4-deoxychorismate lyase | 4.1 |
| A1S_1634 | Rrf2 family transcriptional regulator | 4.1 |
| Phosphate transport | ||
| A1S_2448 | Phosphate transport system substrate-binding protein | 10.5 |
| A1S_2447 | Phosphate transport system permease protein | 6.8 |
| A1S_3374 | Positive Pho regulon response regulator | 6.0 |
| A1S_3376 | Phosphate regulon sensor histidine kinase PhoR | 4.7 |
| A1S_0256 | Phosphate transport system protein | 4.4 |
| Nucleic acid synthesis and repair | ||
| A1S_2273 | RNA 3′-terminal phosphate cyclase (ATP) | 26.4 |
| A1S_2586 | dGTP triphosphohydrolase | 8.6 |
| A1S_0310 | Excinuclease ABC subunit C | 6.4 |
| A1S_1173 | DNA polymerase V | 5.3 |
| A1S_2008 | DNA polymerase V | 4.8 |
| A1S_0636 | DNA polymerase V | 4.8 |
| A1S_1174 | DNA polymerase V | 4.6 |
| A1S_1962 | Recombination protein RecA | 4.4 |
| A1S_3295 | Excinuclease ABC subunit A | 4.2 |
| A1S_3359 | Topoisomerase IV subunit B | 4.1 |
| Amino acid transport and metabolism | ||
| A1S_1443 | Taurine transport system ATP-binding protein | 222.8 |
| A1S_0023 | Putative malic acid transport protein | 135.0 |
| A1S_1397 | Polar amino acid transport system permease protein | 102.2 |
| A1S_1444 | Taurine transport system permease protein | 87.3 |
| A1S_1442 | Taurine transport system substrate-binding protein | 85.9 |
| A1S_1396 | Polar amino acid transport system permease protein | 76.5 |
| A1S_1445 | Taurine dioxygenase | 41.8 |
| A1S_1398 | Polar amino acid transport system ATP-binding protein | 27.1 |
| A1S_0921 | Arginine:ornithine antiporter/lysine permease | 14.7 |
| A1S_1399 | Polar amino acid transport system substrate-binding protein | 11.4 |
| A1S_1485 | d-Methionine transport system substrate-binding protein | 9.9 |
| A1S_2384 | Lysine N6-hydroxylase | 6.6 |
| A1S_1046 | Lysine exporter | 6.3 |
| A1S_1407 | Serine O-acetyltransferase | 6.1 |
| A1S_1400 | Polar amino acid transport system substrate-binding protein | 4.8 |
| Ribosome | ||
| A1S_2271 | tRNA-splicing ligase RtcB | 118.6 |
| A1S_1961 | Ribosome-associated heat shock protein Hsp15 | 5.1 |
| Fatty acid metabolism | ||
| A1S_1436 | Putative acyl-CoA dehydrogenase | 7.8 |
| A1S_1437 | Putative acyl-CoA dehydrogenase | 7.7 |
| A1S_2458 | Linoleoyl-CoA desaturase | 7.5 |
| A1S_0394 | Putative acyl-CoA dehydrogenase | 7.4 |
| Transport | ||
| A1S_1456 | Chromate transporter | 23.1 |
| A1S_1457 | Chromate transporter | 13.1 |
| A1S_2378 | Putative ABC transporter | 8.4 |
| A1S_1720 | NitT/TauT family transport system substrate-binding protein | 8.2 |
| A1S_3272 | MFS transporter, YNFM family, putative membrane transport protein | 5.7 |
| A1S_2377 | Putative ABC transporter | 5.4 |
| A1S_2311 | ABC-2 type transport system ATP-binding protein | 4.4 |
| A1S_1772 | MFS transporter, DHA2 family, multidrug resistance protein | 4.2 |
| Metabolism (other) | ||
| A1S_1488 | Putative acyl-CoA dehydrogenase | 100.9 |
| A1S_1487 | Putative acyl-CoA dehydrogenase | 30.0 |
| A1S_3305 | FMN reductase | 24.6 |
| A1S_1486 | Putative monooxygenase (DszA-like) | 24.5 |
| A1S_0922 | Putative homocysteine S-methyltransferase family protein | 19.9 |
| A1S_0393 | Putative acyl-CoA dehydrogenase | 15.3 |
| A1S_0024 | Adenosylhomocysteine nucleosidase | 10.9 |
| A1S_0463 | Putative alkaline phosphatase | 9.0 |
| A1S_2459 | Putative oxidoreductase | 8.3 |
| A1S_3222 | Homocysteine synthase | 6.9 |
| A1S_2293 | Ferredoxin/flavodoxin–NADP+ reductase | 5.5 |
| A1S_1446 | Allantoin racemase | 4.4 |
| A1S_0131 | Streptomycin 3"-adenylyltransferase | 4.3 |
| Other | ||
| A1S_1717 | GntR family transcriptional regulator | 32.1 |
| A1S_1414 | LrgB-like protein | 27.5 |
| A1S_0466 | Sec-independent protein translocase protein TatA | 25.0 |
| A1S_1665 | Putative membrane protein | 19.9 |
| A1S_3114 | CBS domain-containing membrane protein | 19.0 |
| A1S_1677 | Putative porin precursor | 17.5 |
| A1S_3381 | AnkB protein | 17.4 |
| A1S_0465 | Sec-independent protein translocase protein TatB | 16.1 |
| A1S_1963 | Regulatory protein | 13.2 |
| A1S_0464 | Sec-independent translocation protein TatC | 12.8 |
| A1S_1928 | Putative signal peptide | 12.4 |
| A1S_2473 | Transcriptional regulator, LysR family | 10.4 |
| A1S_1224 | Transposase | 8.9 |
| A1S_0416 | Putative transcriptional regulator (LysR family) | 5.9 |
| A1S_3326 | Putative membrane protein | 5.4 |
| A1S_1438 | Putative coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase | 5.2 |
| A1S_3375 | Phosphate regulon response regulator PhoB | 5.2 |
| A1S_1539 | ArsR family transcriptional regulator | 4.7 |
| A1S_1767 | Putative acid phosphatase | 4.7 |
| A1S_1503 | Transmembrane pair | 4.5 |
| A1S_3360 | Putative esterase | 4.3 |
| A1S_2319 | Putative membrane protein | 4.2 |
| A1S_0565 | Putative membrane protein | 4.2 |
| A1S_1197 | Putative extracellular nuclease | 4.1 |
| Hypothetical | ||
| A1S_0517 | Hypothetical protein | 139.3 |
| A1S_3813 | Hypothetical protein | 73.1 |
| A1S_3666 | Hypothetical protein | 47.7 |
| A1S_3906 | Hypothetical protein | 43.9 |
| A1S_1718 | Hypothetical protein | 30.1 |
| A1S_2272 | Hypothetical protein | 26.2 |
| A1S_3783 | Hypothetical protein | 21.5 |
| A1S_3116 | Hypothetical protein | 15.4 |
| A1S_3115 | Hypothetical protein | 14.6 |
| A1S_3668 | Hypothetical protein | 13.6 |
| A1S_3804 | Hypothetical protein | 12.5 |
| A1S_0617 | Hypothetical protein | 12.2 |
| A1S_3782 | Hypothetical protein | 9.5 |
| A1S_3913 | Hypothetical protein | 8.1 |
| A1S_0889 | Hypothetical protein | 8.0 |
| A1S_1390 | Hypothetical protein | 7.7 |
| A1S_0976 | Hypothetical protein | 7.0 |
| A1S_2266 | Hypothetical protein | 6.9 |
| A1S_3113 | Hypothetical protein | 5.9 |
| A1S_0224 | Hypothetical protein | 5.6 |
| A1S_1614 | Hypothetical protein | 5.5 |
| A1S_3878 | Hypothetical protein | 5.5 |
| A1S_3479 | Hypothetical protein | 5.2 |
| A1S_3780 | Hypothetical protein | 5.1 |
| A1S_3682 | Hypothetical protein | 5.1 |
| A1S_2267 | Hypothetical protein | 4.9 |
| A1S_3912 | Hypothetical protein | 4.9 |
| A1S_3638 | Hypothetical protein | 4.7 |
| A1S_3849 | Hypothetical protein | 4.1 |
| A1S_0770 | Hypothetical protein | 4.1 |
| A1S_3513 | Hypothetical protein | 4.1 |
a
CoA, coenzyme A; MFS, major facilitator superfamily; CBS, cystathionine β-synthase; FMN, flavin mononucleotide.