FIG 3.

Oxidative stress increases the importance of MntABC. (A to C) S. aureus wild-type, ΔmntC, ΔmntH, and ΔmntC ΔmntH cells were prestarved for metals, and growth assays were performed in rich medium in the presence of increasing concentrations of CP and in the absence (A) and the presence of 0.1 mM PQ (B) and 1.0 mM PQ (C). (D and E) Growth assays were performed in the presence of increasing concentrations of CP for wild-type and ΔmntC strains containing either pOS1 plgt (plgt) or pOS1 plgt:mntC (plgt:mntC) in the presence of 0.1 mM PQ (D) or 1.0 mM PQ (E). * = P ≤ 0.05 (two-way ANOVA with Tukey’s posttest corrected for repeated measurements). n ≥ 3. Error bars indicate SEM.