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Alkaline environments increase the importance of MntABC to staphylococcal growth. Growth assays were performed in the presence of increasing concentrations of CP with S. aureus wild-type, ΔmntC, ΔmntH, and ΔmntC ΔmntH strains in defined medium containing glucose as a carbon source and adjusted to pH 6.4 (A), pH 7.4 (B), and pH 8.4 (C). * = P ≤ 0.05 (two-way ANOVA with Tukey’s posttest corrected for repeated measurements). n ≥ 3. Error bars indicate SEM.
