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. 2018 Dec 7;9:2891. doi: 10.3389/fimmu.2018.02891

Table 1.

Analyses of splenocytes and determination of lymphocytes and myeloid cells by flow cytometry at parasitemia between 15 and 20%.

Young (Average values) Old (Average values)
Cells Total No Percentage Cells Total No Percentage t-test Young vs. Old, p-values
N40 Splenocytes 89446.8 89.4 Splenocytes 89891.0 89.9
F4/80 16187.0 18.1 F4/80 7832.3 8.71 0.0286(*)
Nk1.1/FcR+ 38793.6 43.4 Nk1.1/FcR+ 36438.8 40.5 0.0407(*)
CD19 2548.6 2.83 CD19 12855.7 14.3 <0.0001(****)
CD3 6299.6 7.04 CD3 4073.3 4.53 0.0117(*)
CD8a 1311.2 1.46 CD8a 1661.3 1.85 0.7278(NS)
CD4 2333.4 2.59 CD4 3780.0 4.20 0.2127(NS)
N40+Bm Splenocytes 90755.6 90.8 Splenocytes 92252.6 92.3
F4/80 26300.0 28.9 F4/80 6901.3 7.48 <0.0001(****)
Nk1.1/FcR+ 37486.8 41.3 Nk1.1/FcR+ 38820.0 42.1 0.2876(NS)
CD19 2742.0 3.02 CD19 7679.0 8.32 0.0063(**)
CD3 10599.0 11.7 CD3 3091.3 3.35 <0.0001(****)
CD8a 2225.8 2.45 CD8a 1750.3 1.89 0.4594(NS)
CD4 4459.4 4.91 CD4 3062.5 3.32 0.5896(NS)
Bm Splenocytes 90480.2 90.5 Splenocytes 98637.2 98.6
F4/80 25000.6 27.6 F4/80 9461.5 9.59 0.0008(***)
Nk1.1/FcR+ 37720.8 41.7 Nk1.1/FcR+ 41822.2 42.4 0.1853(NS)
CD19 3107.8 3.44 CD19 11047.4 11.2 0.0130(*)
CD3 9758.7 10.8 CD3 3915.9 3.97 0.0001(***)
CD8a 2104.0 2.32 CD8a 1361.2 1.38 0.0716(NS)
CD4 4192.4 4.64 CD4 3166.3 3.21 0.1251(NS)
Naïve Splenocytes 82605.6 82.6 Splenocytes 85431 85.4
F4/80 6436.7 7.79 F4/80 5007 5.86
Nk1.1/FcR+ 30209.4 36.5 Nk1.1/FcR+ 15922 28.7
CD19 1876.0 2.26 CD19 4268 4.99
CD3 3507.7 4.24 CD3 4678 5.47
CD8a 837.3 1.01 CD8a 1364 1.59
CD4 3319.2 4.02 CD4 2857 3.34

Statistical analyses: (

*

p < 0.05,

**

p < 0.01,

***

p < 0.001,

****

p < 0.0001, NS-Not significant).