Figure 3.

Development of the Bivalent Tandem CARs

(A) Schematic of TanCAR structures. (B) Flow-cytometric plot demonstrating the surface binding of CD22Fc and CD19 idiotypes. (C) Cytokine production by CD19-CAR-, CD22-CAR-, TanCAR1-, and TanCAR4-expressing T cells co-incubated with K562, K562-CD19, K562-CD22, and K562-CD19CD22 cell lines. (D) Comparison of in vivo efficacy of TanCAR1 and TanCAR4 CAR T cells. NSG mice were challenged with 1E−6 luciferase-expressing NALM6 leukemia cells on day 0. On day 3, mice were i.v. injected with 3E−6 tandem-CAR-expressing T cells. Quantification of luminescence is shown on the right. ****p < 0.0001. ns, not significant.