Figure 3.

Amino acid sequence alignment of PGDH form representative species. Mt, Mycobacterium tuberculosis; Dr, Danio rerio (zebrafish); Rn, Rattus norvegicus; Hs, Homo sapiens; Sc1 and Sc2, two variants from Saccharomyces cervisiae; Ec, Escherichia coli; Eh, Entamoeba hystolytica; Dm, Drosophila melanogaster. The numbering designates residue position in the figure rather than the sequence of a particular PGDH. The PGDH type is shown as a numeral in front of the species abbreviation. The domains are highlighted with colored lines. Substrate binding domain, blue; nucleotide binding domain, yellow; ASB domain, green; and ACT domain, orange. Conserved residues are highlighted in yellow. Residues involved in l-serine binding at the ACT site are highlighted in green. The active site lysine in some type 3 enzymes is highlighted in magenta. Asterisks designate residues involved in substrate and effector binding and the plus sign identifies the active site histidine or lysine. The carboxyl termini are designated “Ct”.