Skip to main content
. 2018 Jun 27;27(12):1731–1743. doi: 10.1177/0963689718779364

Figure 4.

Figure 4.

APN activates JAK2/STAT3 signaling, increases the ratio of Bcl-2 to Bax, and decreases cleaved-caspase-3 in OGD. HT22 cells were exposed to OGD following 4 h of treatment with APN (25, 50, or 100 µmol/L). (a) Effects of APN on phosphorylation of JAK2 and STAT3 were evaluated using western blot analysis. β-actin was used as a loading control, and the levels of total JAK2, p-JAK2 (Y1007/Y1008), total STAT3, and p-STAT3 (Y705) were normalized to control. (b) Nuclear and cytoplasmic STAT3 were extracted using the nuclear protein extraction kit and evaluated using western blot analysis. Histone H3 and β-actin were used as a loading control, and the levels of nuclear and cytoplasmic STAT3 were normalized to control. (c) Bcl-2, Bax, and cleaved-caspase-3 were evaluated using western blot analysis, which were normalized to control, and β-actin was used as a loading control. n = 6. *P < 0.05, compared with control. # P < 0.05, compared with OGD. & P < 0.05, compared with OGD + 25 µmol/L APN.