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. 2018 Jun 27;27(12):1731–1743. doi: 10.1177/0963689718779364

Figure 6.

Figure 6.

AG490 inhibits JAK2/STAT3 signaling and reverses the effects of APN on the ratio of Bcl-2 to Bax and the level of cleaved-caspase-3. HT22 cells were incubated with 50 µmol/L AG490 for 16 h in advance, and then treated with APN, followed by exposure to OGD. (a) Phosphorylation levels of JAK2 and STAT3 were evaluated using western blot analysis. β-actin was used as a loading control, and the data in the OGD group were defined as 1.0. (b) Nuclear and cytoplasmic STAT3 were extracted using the nuclear protein extraction kit and evaluated using western blot analysis. Histone H3 and β-actin were used as a loading control, respectively, for nuclear and cytoplasmic proteins, and the nuclear translocation of STAT3 in the OGD group was defined as 1.0. (c) Bcl-2, Bax, and cleaved-caspase-3 were evaluated using western blot analysis, which were normalized to the OGD group, and β-actin was used as a loading control. n = 6. # P < 0.05, compared with OGD. & P < 0.05, compared with AG490 + OGD. $ P < 0.05, compared with APN + OGD.