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. 2018 Dec 20;16:103. doi: 10.1186/s12951-018-0429-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Isolation and Characterization of siGRP78 modified MSCs derived from human bone marrow. a Flow cytometric analysis showed BM-MSCs were positive for mesenchymal lineage markers (CD73, CD90 and CD105), negative for hematopoietic and endothelial markers (CD34, CD11b, CD19, CD45), and negative for HLA-DR. b Representative morphology of BM-MSCs. c Down-regulating the expression of GRP78 in BM-MSCs do not influence the stemness trait of MSC. Blue was the control siRNA; red was the siGRP78. d qPCR showed GRP78 was down-regulated in the siGRP78 transfected BM-MSCs compared with control siRNA (scramble siRNA)