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. Author manuscript; available in PMC: 2018 Dec 20.
Published in final edited form as: Biochem Biophys Res Commun. 2016 May 24;476(4):293–298. doi: 10.1016/j.bbrc.2016.05.115

Fig. 1. Mouse hepatic Gadd45b mRNA induction by Wy-14,643 does not correlate with Gadd45b-promoter luciferase assay.

Fig. 1.

(A) Mice were fed with Wy-14,643 (0.1%, w/w) for 1 day and liver samples subjected to qPCR. (B) For in vivo Gadd45b-luciferase assay, Gadd45b promoter (2 Kb) was cloned into the modified pGL4.10 vector. (C) In vivo luciferase activity was measured using bioluminescent imaging system in the mice (Sv129 background). Mice were injected with 10 μg of pGL4.10-Gadd45b (2 Kbp inserted) plasmid for 2 days using the hydrodynamic tail vein injection method and followed by 24 h Wy-14,643 (0.1%, w/w) diet administration. Con, Control; pLuc, pGL4.10 vector; pLuc-2K, pGL4.10-Gadd45b-promoter (2 Kbp); Wy, Wy-14,643; ***, p < 0. 0001.