Fig. 8.

The absence of tubular protective effects of the pretreatment using clofibrate or pioglitazone in Ppara-null mice with FAON. (A–G) Alterations of pathologic findings, tubular damage, kidney function, fatty acid metabolism ability, oxidative stress, apoptosis and inflammation via pretreatment of clofibrate or pioglitazone in Ppara-null mice with FAON, respectively. Ppara-null mice were fed a 0.1% clofibrate-containing or 0.05% pioglitazone-containing diet from two weeks before FFAs-binding BSA injections. The start time point of FFAs-binding BSA injection is designated as day 0. A reduced amount of FFAs-binding BSA (0.325 g/day) was administered to each group for 4 days. The assessment procedure for disease developmental factors was the same as in the clofibrate experiment using wild-type (WT) mice. Data for the regular-diet WT mice, regular-diet Ppara-null mice, clofibrate-diet Ppara-null mice, and pioglitazone-diet Ppara-null mice at days 0 and 4 are indicated. Values represent means±SD (n = 3 for each group at day 0; n = 5 for each group at day 4).