Table 3.
Selection of Identified α7-nAChR Interacting Proteins Associated with Human Diseasea
| protein name (gene name, accession number) | unique Peptides | seq cov. (%) | ion score | prob. Score (%) | AD | IR | PD |
|---|---|---|---|---|---|---|---|
| 5′-AMP-activated protein kinase catalytic subunit alpha-1 (Prkaa1, Q5EG47) | 2 | 6 | 68 | 96 | 47 | 67 | 81 |
| acetyl-CoA acetyltransferase, cytosolic (Acat2, Q8CAY6) ♦ | 2 | 9 | 80 | 100 | - | 62 | 91 |
| ATP synthase-coupling factor 6, mitochondrial (Atp5j, P97450) | 2 | 27 | 102 | 100 | 92 | - | - |
| BDNF/NT-3 growth factors receptor (Ntrk2, P15209) ♦ | 5 | 10 | 225 | 100 | - | - | 80 |
| chondroitin sulfate proteoglycan 5 (Cspg5, Q71M36) | 1 | 2 | 68 | 98 | 93 | - | - |
| CUB and sushi domain-containing protein 1 (Csmd1, Q923L3) | 3 | 1 | 98 | 99 | 94 | - | - |
| disks large-associated protein 3 (Dlgap3, Q6PFD5) ♦ | 1 | 2 | 53 | 99 | - | - | 75 |
| endosomal/lysomomal potassium channel TMEM175 (Tmem175, Q9CXY1) ♦ | 1 | 4 | 58 | 100 | - | - | 95 |
| exocyst complex component 2 (Exoc2, Q9D4H1) | 2 | 2 | 101 | 98 | 96 | - | - |
| gelsolin (Gsn, P13020) ♦ | 4 | 11 | 97 | 100 | 97 | - | - |
| glia maturation factor β (Gmfb, Q9CQI3) ♦ | 2 | 24 | 112 | 100 | - | - | 98 |
| heme oxygenase 2 (Hmox2, O70252) ♦ | 4 | 19 | 150 | 100 | - | - | 79 |
| hyaluronan and proteoglycan link protein 1 (Hapln1, Q9QUP5) | 2 | 8 | 94 | 98 | 99 | - | - |
| insulin-degrading enzyme (Ide, Q9JHR7) | 3 | 3 | 110 | 98 | 100 | 57 | 82 |
| leucine-rich repeat neuronal protein 1 (Lingo1, Q9D1T0) ♦ | 3 | 7 | 113 | 99 | - | - | 78 |
| MAP/microtubule affnity-regulating kinase 2 (Mark2, Q05512) | 6 | 11 | 177 | 100 | 49,50 | - | 101 |
| MAP/microtubule affnity-regulating kinase 4 (Mark4, Q8CIP4) ♦ | 3 | 6 | 91 | 96 | 49 | - | - |
| neuronal acetylcholine receptor subunit alpha-7 (Chrna7, P49582) | 1 | 4 | 67 | 100 | 16,17,41,84 | 102 | 43,103 |
| neuropilin-1 (Nrp1, P97333) ♦ | 1 | 2 | 67 | 100 | 104 | 58 | - |
| nucleobindin-1 (Nucb1, Q02819) ♦ | 5 | 13 | 172 | 100 | 53 | - | - |
| oxysterol-binding protein 1 (Osbp, Q3B7Z2) ♦ | 1 | 4 | 81 | 100 | 105 | - | - |
| potassium voltage-gated channel subfamily A member 3 (Kcna3,P16390) | 5 | 11 | 266 | 100 | 55,106 | 59,60 | - |
| prelamin-A/C (Lmna, P48678) ♦ | 3 | 6 | 157 | 100 | - | 64 | - |
| protein kinase C δ type (Prkcd, P28867) ♦ | 1 | 2 | 52 | 99 | 51 | 65,66 | - |
| Ras/Rap GTPase-activating protein SynGAP (Syngap1, F6SEU4) | 2 | 3 | 76 | 97 | 105 | - | - |
| regulator of G-protein signaling 6 (Rgs6, Q9Z2H2) | 3 | 10 | 159 | 100 | 56 | - | - |
| Sec24 related gene family, member D (Sec24d, Q6NXL1) | 2 | 3 | 98 | 100 | - | - | 107,108 |
| target of Myb protein 1 (Tom1, O88746) | 2 | 5 | 83 | 95 | 109 | - | - |
| utrophin (Utrn, E9Q6R7) | 3 | 1 | 139 | 100 | 110,111 | 112 | 113 |
| voltage-dependent anion-selective channel protein 2 (Vdac2, Q60930) | 2 | 8 | 107 | 100 | 114 | - | - |
| zyxin (Zyx, Q62523) ♦ | 1 | 3 | 66 | 100 | 115 | - | - |
a
Proteins associated with Alzheimer’s disease (AD), insulin resistance (IR), and/or Parkinson’s disease (PD). These interactions are related to these pathologies either directly by the protein or through genetic association. Proteins are identified by protein name, gene name, and accession numbers. The number of unique peptides, sequence coverage (seq. cov. %), ion scores, probability (prob. score %), and the associated disease are all indicated. High-salt-wash-eluted proteins are identified with “♦.” AD, IR, and PD columns provide references for examples of associations of each protein with the specified disease state.