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. Author manuscript; available in PMC: 2018 Dec 20.
Published in final edited form as: Oncogene. 2015 Feb 23;34(44):5548–5559. doi: 10.1038/onc.2015.10

Figure 1.

Figure 1.

Expression of miR-217 in cultured IEEC and EACC. (*p<0.05; **p<0.01)

A: qRT-PCR analysis of endogenous miR-217 expression normalized with control miRNA (RNU44) in IEEC and EACC. Basal levels of miR-217 were significantly lower in in EACC relative to IEEC.

B: qRT-PCR analysis demonstrating repression of miR-217 in IEEC and EACC following 5 day CSC exposure.

C and D: qRT-PCR and immunoblot analysis demonstrating up-regulation of KLK7 expression in EACC relative to IEEC.

E: q-PCR analysis of KLK7 expression in Het-1A, EsC1, EsC2, and OE19 cells over-expressing precursor or antisense miR-217 constructs. CSC enhanced KLK7 expression particularly in EACC. Over-expression of miR-217 significantly attenuated CSC-mediated induction of KLK7.