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. Author manuscript; available in PMC: 2018 Dec 20.
Published in final edited form as: Oncogene. 2015 Feb 23;34(44):5548–5559. doi: 10.1038/onc.2015.10

Figure 7.

Figure 7.

NF1 mediates CSC-induced repression of miR-217. (**p<0.01)

A: q-PCR analysis of NF1 expression in Het-1A, CP-A, CP-C, EsC1, EsC2, and OE19 cells. NF1 levels were lower in EACC relative to Het-1A, CP-A, and CP-C. CSC treatment had no significant effect on NF-1 expression in these cells.

B: q-PCR analysis demonstrating that siRNA-mediated knock-down of NF1 significantly diminishes miR-217 expression in Het-1A, and to a lesser extent in EsC1, EsC2, and OE19 cells.

C: Quantitative ChIP analysis of NF1 occupancy within the miR-217 associated CpG island in Het-1A, EsC1, EsC2, and OE19 cells cultured with or without CSC. Cigarette smoke markedly decreases occupancy of NF1 within this CpG island.