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. Author manuscript; available in PMC: 2018 Dec 20.
Published in final edited form as: Oncogene. 2015 Feb 23;34(44):5548–5559. doi: 10.1038/onc.2015.10

Figure 8.

Figure 8.

TGF-β1 mediates repression of miR-217 via de novo methylation. (*p<0.05; **p<0.01)

A: qRT-PCR analysis demonstrating that CSC exposure (48h) increased TGF-β1 expression in Het-1A, EsC1, EsC2, and OE19 cells.

B: qRT-PCR analysis demonstrating that 48h exposure to recombinant TGF-β1 decreased miR-217 expression in Het-1A and EACC.

C: qRT-PCR analysis demonstrating that a neutralizing TGF-β1 antibody modestly increased miR-217, but not miR-216a expression in Het-1A, EsC1, EsC2, and OE19 cells.

D: MeDIP analysis demonstrating that recombinant TGF-β1 increased DNA methylation levels in Het-1A cells at 5ng/ml, and in EsC1 and EsC2 cells at 10ng/ml level. This neutralizing antibody had no effect on DNA methylation within UBE2B or H19 ICR.