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. Author manuscript; available in PMC: 2019 Apr 16.
Published in final edited form as: Mucosal Immunol. 2018 Oct 16;12(1):200–211. doi: 10.1038/s41385-018-0091-7

Figure 1. Regulatory T cell expression during ileitis.

Figure 1.

(A) Flow cytometric analysis of the frequency of CD4+FoxP3+ from the spleen (SP), mesenteric lymph node (MLN) and ileal lamina propria (LP) demonstrate a significant increase in Treg frequency at peak disease (8–12 weeks) in TNFΔARE/+ mice relative to WT. (B) The increased frequency was offset by a reduction in IL-10 producing CD4+FoxP3+ Tregs in ilea of 8wk TNFΔARE/+ mice relative to WT littermates. (C) Treg accumulation was confirmed by Taqman real-time PCR measurement of the relative FoxP3 mRNA expression in ileal whole tissue with results expressed relative to 18S or CD4. (D) PCR analysis of isolated CD4+CD25+ Tregs from WT and TNFΔARE/+ mice indicated no significant difference in IL-10 mRNA. Results represent mean ± SEM for three mice per group from three independent studies. *P<0.05, **P<0.01, ***P<0.01.