Fig. 5.

Growth factors secreted by PBECs prime the PDC responses to influenza. Purified PDCs were stimulated with influenza in the presence of growth factors, GMCSF, GCSF, VEGF or a cocktail of three. a Bar graphs depict the cytokines secreted by PDCs as assayed by magpix assay. Data is mean +/− S.E. of five experiments with PDCs from different subjects. b PDCs were cultured with PBEC conditioned medium and stimulated with influenza (Flu) in the presence of antibodies to GMCSF, GCSF, VEGF (α-GMCSF, α-GCSF, α-VEGF) or a combination of all three antibodies (α−3). Bar graphs depict percent inhibition of the cytokines secreted by PDCs. Data is mean +/− S.E. of five experiments of which three experiments were done where PDCs from three different subjects were cultured with PBEC conditioned media from one set of PBEC and the other two experiments were done where PDCs from another two subjects were cultured with conditioned media from second set of PBEC