. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: Mol Diagn Ther. 2018 Aug;22(4):443–457. doi: 10.1007/s40291-018-0343-y

Table-2.

Summary of the comparative analytical sensitivity of real time PCR assay targeting leishmania DNA region

Target Sequence	Tissue tested	Assay Chemistry	Analytical Sensitivity (parasite/ml or parasite DNA equivalence/reaction)	Reference
kDNA	Blood	Intercalating dye	0.07-0.1	[130]
	Blood	Fluorescent dye	0.001-0.002	[131, 132]
	Bone Marrow/Lymph Nodes	Intercalating eye	0	[133]
	Bone Marrow/Lymph Nodes	Fluorescent dye	0.001-0.0125	[66, 132]
	Skin Biopsy	Intercalating dye	0.0001-0.5	[134, 135]
	Skin Biopsy	Fluorescent dye	0.005	[136]
rDNA	Blood	Intercalating dye	0.5-10.0	[137, 138]
	Blood	Fluorescent dye	1.0 – 6.25	[139, 140]
	Bone Marrow/Lymph Nodes	Intercalating eye	0.1 – 10.0	[138, 141]
	Bone Marrow/Lymph Nodes	Fluorescent dye	1.0	[140]
	Skin Biopsy	Intercalating dye	0.5 – 10.0	[137, 138]
	Skin Biopsy	Fluorescent dye	1.0 – 1000.0	[64, 140]
GPI	Skin biopsy	Fluorescent dye	165.4	[142]
G6PD	Biopsy	Intercalating dye	0.005	[136]
HSP70	Parasite culture	Intercalating dye	10.0	[143]
cpb	Biopsy	Fluorescent dye	0.01	[144]

Abbreviations

G6PD: Glucose-6-phosphate dehydrogenase; HSP70: Heat-shock protein 70; kDNA: Kinetoplast DNA; rDNA: ribosomal DNA; cpb: cystein protease B; GPI: Glucose phosphate isomerase; qPCR: Quantitative real-time PCR