Figure 1. cDC2 play a central role in mediating the adjuvant activity of CDG.

A. Flow cytometry analysis of MPYS expression in lung DC subsets. n>3. B. Absolute number of lung DC subsets in C57BL/6 mice administered (i.n.) with saline or 5µg FITC-CDG for 5 hours. n=3.C. Absolute number of CD86⁺ lung DC in mice administered (i.n.) with saline or 5µg CDG for 16 hours. n>3. D. Absolute number of CCR7⁺ lung DC in mice administered (i.n.) with saline or 5µg CDG for 16 hours. n>3. E-G. Flow cytometry analysis of pRelA and pRelB in cDC1 (E), cDC2 (F), and moDCs (G) from mice treated with saline or CDG for 16hrs. n>3. H. C57BL/6, Batf3^−/−, and IRF4^fl/flCD11c^cre mice were immunized (i.n.) with two doses (14 days apart) of PspA or PspA plus CDG (5ug). Anti-PspA IgG in serum and IgA in BALF were determined by ELISA. n>3. I. IRF4^fl/flCD11c^cre mice were adoptively transferred (i.n.) with lung cDC2 sorted from WT mice lung and immunized (i.n.) with PspA or CDG/PspA. Serum anti-PspA IgG and BALF IgA were determined by ELISA. n=3. Graphs represent means ± standard error from three independent experiments. The significance is represented by and asterisk (*) where p<0.05 (unpaired Student’s t test).