Figure 5. Lung cDC2 consist of two distinct subpopulations.

A. Flow cytometry analysis of lung TNFR2+ vs TNFR2− cDC2 at steady-state. n=3. B-C. Flow cytometry analysis of IRF4 and IRF8 (B) and Zbtb46 (C) in TNFR2+ and TNFR2− cDC2. n=3. D. Flow cytometry analysis in lungs of IRF4fl/flCD11ccre mice reconstituted with CD45.1+ pre-cDC2. n=3. pre-cDC2 were sorted from the bone marrow of B6.CD45.1 mice and transferred (i.n.) into IRF4fl/flCD11ccremice. n=3. E. Phenotypic analysis of CD45.1 TNFR2+ and TNFR2− cDC2 transferred into IRF4fl/flCD11ccre mice. n=3. F. Flow cytometry analysis of TNFR2 expression on cDC2 subpopulations transferred into MPYS−/− mice treated with CDG (i.n.) for 16hrs. n=3.