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. 2019 Feb;14(2):361–368. doi: 10.4103/1673-5374.244799

Figure 1.

Figure 1

Western blot assay of γ-H2A.X protein expression in SBA embryos and controls.

(A–D) Western blot results of γ-H2A.X protein expression in individual embryos on E11, E14, E16, and E18. There was a significant difference in the relative protein expression of γ-H2A.X in SBAs and controls on E14. *P < 0.05, vs. control. Data are represented as the mean ± SEM (n = 3 per group at each time point; Student’s t-test). MGMT: O6-methylguanine DNA methyltransferase; SBA: spina bifida aperta; E: embryonic day.