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Methylation status analysis of the MGMT promoter in SBA and control animals on E18.
Methylation analysis of the MGMT promoter in E18 spinal cords (n = 5) by BSP in normal control and SBA animals. Each of lines represents a single clone. ●: Methylated CpGs; ○: unmethylated CpGs; N: control; S: SBA. MGMT: O6-methylguanine DNA methyltransferase; SBA: spina bifida aperta; BSP: bisulfite sequencing PCR; E: embryonic day.
