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. 2018 Dec 13;9:1852. doi: 10.3389/fpls.2018.01852

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Copyright © 2018 Abd-El-Haliem, Hoogstrate and Schuurink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Successful expression of GFP in tomato leaf discs after vacuum infiltration with Agrobacterium tumefaciens and recovery from infiltration. Leaf discs prepared from 3.5 to 4-week-old tomato plants of the cultivar Moneymaker were vacuum-infiltrated with the Agrobacterium tumefaciens strain 1D1249 carrying the plasmid pGWB512 in which the eGFP is expressed under the control of the 35S promoter of cauliflower mosaic virus. Leaf discs were allowed to recover from the infiltration for 1 h under an air stream and then placed on water agar in 6-well plates. Pictures were taken using a wide-field fluorescence microscope at the indicated time points and magnification.