Fig. 4. HEK Flp-in wt cells transiently transfected with pplss-3HA-hCB2 or mock transfection, preincubated with SR144528 or vehicle for 30 min, then treated with 32 + SR144528 or vehicle (2 min followed by 3 washes). Cell surface CB2 receptor visualised using mouse anti-HA and Alexa 488-conjugated goat anti-mouse. Scale bar 10 μM. Images representative of n = 3 experiments.
