Table 1. List of reactions for rhamnolipids biosynthesis added to the P. putida model.

Code	Reaction formula	Reversibility
RHLA	(3R)-3-Hydroxydecanoyl-acyl-carrier protein + Coenzyme A ⇒ (S)-3-Hydroxydecanoyl-CoA + acyl carrier protein	Irreversible
RHLB	3-hydroxydecanoyl-3-hydroxydecanoate + dTDP-4-dehydro-6-deoxy-L-mannose + H+ ⇒ dTDP + L-rhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoate	Irreversible
RHLC	dTDP-4-dehydro-6-deoxy-L-mannose + H+ + L-rhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoate ⇒ dTDP + L-rhamnosyl-Lrhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoate	Irreversible
3H3H	2 beta-hydroxydecanoyl-beta-hydroxydecanoyl-S-CoA + H2O ⇒ 3-hydroxydecanoyl-3-hydroxydecanoate + Coenzyme A	Irreversible
PHAC	(S)-3-Hydroxydecanoyl-CoA ⇒ 2 beta-hydroxydecanoyl-beta-hydroxydecanoyl-S-CoA + Coenzyme A	Irreversible

Note:

Reactions RHLA, RHLB and RHLC represent the rhamnosyltransferase chain A, rhamnosyltransferase chain B and rhamnosyltransferase 2 respectively. Reactions 3H3H and PHAC are involved in the poly(3-hydroxyalkanoic acid) synthase.