Table 2. Contribution of variables and individual reactions on the principal components.
| PC1 | PC2 | PC3 | PC4 | PC5 | |
|---|---|---|---|---|---|
| (A) | |||||
| Glucose | 17.733 | 10.669 | 0.001 | 56.157 | 15.396 |
| Fructose | 19.748 | 4.086 | 0.463 | 4.852 | 3.273 |
| Sucrose | 19.167 | 1.467 | 4.929 | 34.892 | 29.010 |
| Benzoate | 11.431 | 46.868 | 34.473 | 3.889 | 3.100 |
| Glycerol | 18.208 | 10.804 | 0.978 | 0.101 | 48.304 |
| Myristic acid | 13.713 | 26.106 | 59.157 | 0.108 | 0.917 |
| (B) | |||||
| RR08593 | 23.878 | 0.683 | 0.878 | 0.575 | 0.040 |
| RR08674 | 22.980 | 9.956 | 2.130 | 3.316 | 0.376 |
| EX_EC0001 | 17.651 | 7.972 | 2.223 | 0.260 | 0.772 |
| EX_EC0007 | 5.952 | 8.456 | 0.015 | 0.201 | 0.096 |
| IR10022 | 5.758 | 0.401 | 17.861 | 0.124 | 0.569 |
| RR04368 | 0.963 | 9.394 | 10.448 | 0.323 | 0.162 |
Note:
(A) Fructose and sucrose are highly correlated with the first component (PC1), compared to benzoate or myristic acid. This variation is driven by the activity of core metabolic reactions for energy demand toward growth and rhamnolipid synthesis. (B) The individual reactions driving rhamnolipid synthesis scored highly on the first two principal components; these include ATP synthase (reaction id: RR08593), cytochrome-c reductase and succinate dehydrogenase (reaction id: IR10022 and RR04368).