Table 1. Primer and probe sequences of LAMP-AuNP for C. trachomatis detection.
Primer and Probe Name | Sequence (5ʹ-3ʹ) |
---|---|
CT-F3 | GAACAGA(A/T)GC(T/A)GCGACAG |
CT-B3 | (C/T)GGGTTTAGAGTAGT(G/A)A(C/T)ATC |
CT-FIP | TTAACTCCAATGTA(A/G)GGAGTGAACA-ATGCCTCTATTGA(C/T)TACCAT |
CT-BIP | GGTCT(A/C)GAG(T/C)AAGTTTTGATGCCG–CAAGAT(T/A)GCTT(C/T)AGCCAATT |
CT-LF | CATATT(T/C)A(AAT(C/T)CGTATAGCTCAGCC |
CT-LB | AT(C/T)CGTATAGCTCAGCC |
CT-AuNP | SH-AAAAAAAAAAGA(G/A)TGGCAAGCAAGTTTA |
Abbreviations: AuNP, gold nanoparticle probe; B, reverse; CT, Chlamydia trachomatis; F, forward; IP, initial loop; L, loop.