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. 2018 Dec 20;13(12):e0209283. doi: 10.1371/journal.pone.0209283

Fig 2. Isoflurane produces age- and region-dependent changes in IL-1β gene expression.

Fig 2

3 and 20-month-old mice were anesthetized with 1.2% isoflurane in 30% oxygen for 4 h and sacrificed 3 h or 48 h later and IL-1β measured in the hippocampus and frontal cortex by RT-PCR. Isoflurane had no effect in either region in young mice (panels A & C). In contrast, it produced a marked and sustained decrease in IL-1β mRNA in the hippocampus and an increase in the cortex 2 d later in aged mice (panels B & C) demonstrating age and region specific effects of treatment with isoflurane. Data expressed as mean ± SD for N = 6 animals per treatment condition. *** p<0.001 relative to age-matched controls by 1-way ANOVA with Dunnett’s test for multiple comparisons.