Fig 2. Phylogenetic tree of CLCs in diverse plant species.

A total of 118 protein sequences from 10 species including Brassica napus, Brassica rapa, Brassica oleracea, Arabidopsis thaliana, Arabidopsis lyrata, Camelina sativa, Populus euphratica, Raphanus sativus, Tarenaya hassleriana and Theobroma cacao were multi-aligned using the ClustalW program, and then an unrooted phylogenetic tree was constructed using the software MEGA6.06 with the neighbor-joining method. The percentage of replicate trees, in which the associated taxa clustered together in the bootstrap test (1000 replicates), are shown next to the branches.