Fig 5. Biased angiogenesis due to hypoxia.

(A) Representative images from the P (top), CH (middle), and IH (bottom) conditions. In each category, images of the vascular network before (left) and after (right) the condition was applied is shown. (B) The total vessel area for each category in the left and right stromal chambers were measured and compared. For both hypoxic conditions, the vessel area in the left stromal chamber (closer to the scavenger channel) was significantly higher than the right chamber. (C) Biased angiogenesis was calculated for the left and right stromal chambers in each condition and compared to each other. Both hypoxic conditions had significantly more bias in the left stromal chamber. P, n = 8; CH, n = 11; IH, n = 17. Scale bar = 200μm * p < .05, ** p < .005, *** p < .0005, **** p < .0001.