Appendix 1—figure 1. Promoter sequences used in this study.

In all panels, the −35 and −10 hexamers of the RNAP binding site are in bold. CRP binding site centers are indicated by small triangles. The palindromic pentamers of the core CRP binding site in each construct are underlined. The transcription start site (TSS) is bold and italicized. Lowercase bases (‘a’,‘c’,‘g’, and ‘t’) indicate positions synthesized with a 24% mutation rate. The lowercase character ‘n’ indicates completely randomized positions. (A) Occlusion promoters assayed for Figure 2. (B) Class I promoters assayed for Figure 5. In the main text we refer to the wild-type promoter with CRP at −61.5 bp as the lac* promoter. The lac* promoter served as the template for all of the promoters shown here. (C) Strong class I promoters assayed for Figure 8. (D) Class II promoters assayed for Figure 9.